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High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

https://doi.org/10.1038/s41598-019-54126-w

Johansen, Ida Elisabeth ; Liu, Ying ; Jørgensen, Bodil ; Bennett, Eric Paul ; Andreasson, Erik ; Nielsen, Kåre L. ; Blennow, Andreas ; Petersen, Bent Larsen ( November 2019 , Scientific Reports)

Abstract
CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potatoU6promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the ArabidopsisU6promotor, driving expression of the CRISPR component, with endogenous potatoU6promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

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